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Agar comes from multiple sources, as many red seaweeds are “agarophytes” (that is, seaweeds containing agar in their cell walls). Species of Gelidium are the most important source of bacteriological (lab-grade) agar. Other main agarophytes, largely used for culinary agar, include red seaweeds from the genera Gracilaria, Pterocladia, Ahnfeltia, and others. Species from the genera Eucheuma, Gigartina, and Chondrus have been used as agarophytes in research during agar shortages.9
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Agar’s low viscosity also makes it easy to pour into Petri dishes, and its transparency permits observation of microbes growing on its surface.7 Also aiding in this is its low syneresis (extrusion of water from the gel), guaranteeing less surface “sweating”: Once a plate is inoculated, bacterial colonies stay in place and do not mix.
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